ranjan

Cryo-electron microscopy breaks a key barrier that will allow the working of proteins to be probed in unprecedented detail. By achieving Atomic resolution using Cryogenic-electron microscopy (cryo-EM) the working of Proteins that cannot be easily examined by other imaging techniques e.g. X Ray crystallography, be understood. The number of protein structures being determined by Cryo- electron microscopy is growing at an explosive rate.  Cryo-EM is a decades old technique that determines the shape of flash-frozen samples by firing electrons at them and recording the resulting images. Advances in technology for detecting the ricocheting electrons and in image-analysis software catalysed a 'resolution revolution' that started around 2013. This led to protein structures that were sharper than ever before - and nearly as good as those obtained from X Rav crystallography, an older technique that infers structures from diffraction patterns made by protein crystals when they are bombard